Baicalin increases developmental competence of mouse embryos in vitro by inhibiting cellular apoptosis and modulating HSP70 and DNMT expression
نویسندگان
چکیده
Scutellaria baicalensis has been effectively used in Chinese traditional medicine to prevent miscarriages. However, little information is available on its mechanism of action. This study is designed specifically to reveal how baicalin, the main effective ingredient of S. baicalensis, improves developmental competence of embryos in vitro, using the mouse as a model. Mouse pronuclear embryos were cultured in KSOM medium supplemented with (0, 2, 4 and 8 μg/ml) baicalin. The results demonstrated that in vitro culture conditions significantly decreased the blastocyst developmental rate and blastocyst quality, possibly due to increased cellular stress and apoptosis. Baicalin (4 µg/ml) significantly increased 2- and 4-cell cleavage rates, morula developmental rate, and blastocyst developmental rate and cell number of in vitro-cultured mouse embryos. Moreover, baicalin increased the expression of Gja1, Cdh1, Bcl-2, and Dnmt3a genes, decreased the expression of Dnmt1 gene, and decreased cellular stress and apoptosis as it decreased the expression of HSP70, CASP3, and BAX and increased BCL-2 expression in blastocysts cultured in vitro. In conclusion, baicalin improves developmental competence of in vitro-cultured mouse embryos through inhibition of cellular apoptosis and HSP70 expression, and improvement of DNA methylation.
منابع مشابه
The effect of all-trans retinol on in vitro mouse embryo's developmental competence
BACKGROUND: All-trans retinol is a biological antioxidantscavenging the ROS in the cell culture. OBJECTIVES: This studywas conducted to investigate the effect of all-trans retinol infertilization and culture medium on mouse embryo's developmentalcompetence. METHODS: This study was designed into twoexperiments. In the first experiment, in vitro mature oocytes wereco-cultured with sperm in fertil...
متن کاملP-117: Gene Expression and Developmental State of Mouse Cloned Embryos after Treatment with Histone Deacetylase Inhibitor,Suberoylanilide Hydroxamic Acid (SAHA)
Background: It is known that acetylation level of the nuclear histones in cloned embryos is lower compare to normally developed embryos. Histone deacetylas inhibitors (HDACi) with improvement of acetylation level in these embryos can affect embryo quality in pre-implantation stage and expression level of different genes especially developmental genes. Materials and Methods: In this research, SA...
متن کاملP-115: Melatonin Increases Developmental Rate of In Vitro Mouse Somatic Cell Nuclear
Background: The beneficial effect of supplementing culture medium with melatonin has been reported during in vitro embryo development of species such as mouse, bovine and porcine. However, the effect of melatonin on the mouse somatic cell nuclear transfer remained unknown. Materials and Methods: In this study, we assessed the effects of various concentrations of melatonin (10-6 to 10-12 M) on t...
متن کاملP-31: Evaluation of In vitro Fertilization Rate, Embryo Quality and Development in Experimental Model of Diabetes induced by STZ: in Female Mouse
Background: Diabetes mellitus is a metabolic condition characterized by elevated blood glucose levels secondary to absolute impairment of insulin secretion. Maternal diabetes has been demonstrated to adversely affect pre implantation embryo development and pregnancy outcomes. Emerging evidence has implicated that these effects are associated with compromised oocyte competence. Several developme...
متن کاملThe Study on Expression of Mous Oocyte and Preimplantation Embryc Mct1 and Mct3 Genes in Vivo and in Vitro
Purpose: The aim of this study was to assay the profile of MCT1 & MCD in mouse unfertilized & fertilized oocytes and preimplantation embryos In vivo and In vitro. Materials and Methods: The presence of mRNAs encoding MCT1 & MCD3 were determined On unfertilized and fertilized oocytes, 2-cell, morulae, blastocyst and cultured embryos in plus glucose KSOM, minus glucose KSOM and pulse glucose KSO...
متن کامل